Deae dextran chloroquine method

Discussion in 'Canada Drug' started by Index1000, 04-Mar-2020.

  1. MBK Moderator

    Deae dextran chloroquine method


    The soluble complex is added to the cells, which take it up by nonspecific endocytosis. The method described here uses an initial incubation with a DNA/DEAE-dextran mixture, followed by a second incubation in growth medium supplemented with chloroquine.

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    DEAE-dextran was the first non-viral transfection method verified by Vaheri and Pagano in 1965. DEAE-Dextran is a cationic polymer that tightly associates with the negatively charges nucleic acids. principle DNA is mixed with DEAE-dextran DNA/polymer complex comes into contact with negatively charged membrane due to excess of positive charge. DEAE-Dextran Transfection Kit Product Code DE-DEX Technical Bulletin No. MB 335 TECHNICAL BULLETIN Product Description DEAE-dextran transfection is a method for the introduction of DNA into eukaryotic cells. DEAE-dextran mediated transfection of MK cells with SV40 DNA was first described more than 20 years ago.1 In Transfection Mediated by DEAE-Dextran High-Efficiency Method Protocol summary only for purposes of this preview site Transfection mediated by DEAE-dextran differs from calcium phosphate coprecipitation in three important respects. First, it is used for transient expression of cloned genes and not for stable transformation of cells.

    This protocol describes transfection of lymphocyte cell lines, but it can be used for transient transfection of both adherent and nonadherent cells. Chloroquine is thought to enhance transfection efficiencies by neutralizing lysosomal hydrolases, which can degrade the DNA.

    Deae dextran chloroquine method

    DEAE - Dextran Thaler Center for HIV and Retrovirus Research, DEAE-Dextran Transfection Kit DEDEX - Bulletin

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  7. Methods. DEAE dextran and Ca3PO42 precipitation showed the least and the great-est inter-assay variation, respectively. For all other methods, inter-assay variability fell within this range. The results presented may serve as both a general reference and a guide for reporter gene studies in this or other macrophage cell lines. INTRODUCTION

    • Evaluation of Methods for Transient Transfection of a Murine..
    • Transfection Mediated by DEAE-Dextran High-Efficiency Method.
    • THE JOURNAL OF Vol. 264, No. 19, Issue of July 5, pp..

    To examine the localization of mutant sortilin, we transiently transfected COS-7 cells with the pcDNA3.1 vector using a DEAE/Dextran chloroquine phosphate technique. Cells were then incubated for 48 hours to allow the mutant protein to be expressed. The soluble complex is added to the cells, which take it up by nonspecific endocytosis. The method described here uses an initial incubation with a DNA/DEAE-dextran mixture, followed by a second incubation in growth medium supplemented with chloroquine. Chloroquine treatment of rodent cells during the first hours of polyoma DNA transfection increase the fraction of cells expressing viral functions. The effect has been observed after DNA absorption using both the DEAE-dextran and calcium phosphate coprecipitation methods.

     
  8. kolyaba New Member

    Plaquenil (hydroxychloroquine) belongs to a group of medicines called quinolines. Plaquenil - Uses, Side Effects, Interactions - Hydroxychloroquine Plaquenil Side Effects & Dosage for Malaria Side Effects of Plaquenil Hydroxychloroquine, Warnings, Uses
     
  9. L e x x New Member

    Plaquenil & Weight Gain/Loss - After 3 years without any active Lupus symptoms, my rheumy suggested we try doing without the Prednisone. Now I've been totally medication-free for over 2 years. Now that still means low-stress, healthy eating, regular mild exercise, good sleep, and taking a nap 2-4 days a week.

    Quitting Plaquenil after 2 years - Sjogren's World